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| カタログ番号 | HP108012 |
|---|---|
| 説明 |
Recombinant Human SRCAP Protein, N-His (HP108012) expressed in E. coli, spanning Lys2044-Thr2197. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q6ZRS2 |
| タンパク質長 | Lys2044-Thr2197 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Helicase SRCAP, 3.6.4.-, Domino homolog 2, Snf2-related CBP activator, SRCAP, KIAA0309 |
| 背景 | Chromatin remodeling protein SRCAP is a ~343 kDa protein. Acts both as a chromatin remodeler and transcription coregulator. Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes expression through chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription. _x005F_x005F_x000D_ _x005F_x005F_x000D_1. Ruhl, DD. et al. (2006) Biochemistry 45, 5671-7. PMID: 16634648 _x005F_x005F_x000D_
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2. Wong, MM. et al. (2007) The Journal of biological chemistry 282, 26132-9. PMID: 17617668 _x005F_x005F_x000D_
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3. Johnston, H. et al. (1999) The Journal of biological chemistry 274, 16370-6. PMID: 10347196 _x005F_x005F_x000D_
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4. Monroy, MA. et al. (2001) The Journal of biological chemistry 276, 40721-6. PMID: 11522779 _x005F_x005F_x000D_
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5. Monroy, MA. et al. (2003) Molecular endocrinology (Baltimore, Md.) 17, 2519-28. PMID: 14500758 _x005F_x005F_x000D_
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6. Eissenberg, JC. et al. (2005) Molecular and cellular biology 25, 6559-69. PMID: 16024792 _x005F_x005F_x000D_ |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SRCAP Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
