Recombinant Human STAT3 Protein, C-His (HW708011) expressed in Insect Cells, spanning Met1-Met770. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии
Insect cells
Номер доступа
P40763
Длина белка
Met1-Met770
Применения
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
89.04 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF
Фон
Signal transducer and activator of transcription 3 (STAT3) is a ~88 kDa protein. Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
1. Tsai, YT. et al. (2000) Molecular and cellular biology 20, 2043-54. PMID: 10688651
2. Yamamoto, T. et al. (2002) Biochemical and biophysical research communications 297, 811-7. PMID: 12359225
3. Shao, H. et al. (2003) Cancer research 63, 3923-30. PMID: 12873986
4. Dreuw, A. et al. (2004) The Journal of biological chemistry 279, 36112-20. PMID: 15194700
5. Yuan, ZL. et al. (2005) Science (New York, N.Y.) 307, 269-73. PMID: 15653507
6. Ray, S. et al. (2005) Gastroenterology 129, 1616-32. PMID: 16285960
7. Saxena, NK. et al. (2007) The Journal of biological chemistry 282, 13316-25. PMID: 17344214
8. Jiang, H. et al. (2008) Biochemical and biophysical research communications 368, 1-5. PMID: 18242580
9. Hou, T. et al. (2008) The Journal of biological chemistry 283, 30725-34. PMID: 18782771
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