Human STAT4 Recombinant Protein (N-His) (HC549012)

Name: Human STAT4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC549012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	HC549012
Описание	Recombinant Human STAT4 Protein, N-His (HC549012) expressed in E. coli, spanning Tyr505-Ile737. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q14765
Длина белка	Tyr505-Ile737
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	28.84 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Signal transducer and activator of transcription 4, STAT4
Фон	Signal transducer and activator of transcription 4 (STAT4) is a ~85 kDa protein. Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. 1. Visconti, R. et al. (2000) Blood 96, 1844-52. PMID: 10961885 2. Baghdassarian, H. et al. (2023) The New England journal of medicine 388, 2241-2252. PMID: 37256972 3. Cho, SS. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 4781-9. PMID: 8943379 4. Morinobu, A. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 12281-6. PMID: 12213961 5. Zhang, YS. et al. (2022) Cell death and differentiation 29, 2303-2315. PMID: 35614130 6. Yao, BB. et al. (1999) Archives of biochemistry and biophysics 368, 147-55. PMID: 10415122 7. Bacon, CM. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7307-11. PMID: 7638186 8. Oppmann, B. et al. (2000) Immunity 13, 715-25. PMID: 11114383 9. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746 10. Gonzales-van Horn, SR. et al. (2016) European journal of immunology 46, 1504-10. PMID: 26990433
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human STAT4 Protein, N-His

Ссылки

Даташит

Human STAT4 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Volume

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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