Human SUFU Recombinant Protein (N-His) (HP131012)

Name: Human SUFU Recombinant Protein (N-His)
Brand: abinScience
SKU: HP131012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HP131012
Descripción	Recombinant Human SUFU Protein, N-His (HP131012) expressed in E. coli, spanning Gly33-Asn265. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9UMX1
Longitud de proteína	Gly33-Asn265
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	28.51 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	SUFU, Suppressor of fused homolog, SUFUH
Fondo	Suppressor of fused homolog (SUFU) is a ~53 kDa protein. Negative regulator in the hedgehog/smoothened signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Down-regulates GLI2-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. 1. Kogerman, P. et al. (1999) Nature cell biology 1, 312-9. PMID: 10559945 2. Stone, DM. et al. (1999) Journal of cell science 112 ( Pt 23), 4437-48. PMID: 10564661 3. Murone, M. et al. (2000) Nature cell biology 2, 310-2. PMID: 10806483 4. Taylor, MD. et al. (2002) Nature genetics 31, 306-10. PMID: 12068298 5. Clark, HF. et al. (2003) Genome research 13, 2265-70. PMID: 12975309 6. Merchant, M. et al. (2004) Molecular and cellular biology 24, 8627-41. PMID: 15367681 7. Chi, S. et al. (2012) Cellular signalling 24, 1222-8. PMID: 22365972 8. Zhang, Y. et al. (2013) Nature communications 4, 2608. PMID: 24217340 9. Cherry, AL. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2563-79. PMID: 24311597 10. Raducu, M. et al. (2016) The EMBO journal 35, 1400-16. PMID: 27234298
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human SUFU Protein, N-His

Referencias

Datasheet

Human SUFU Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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