

| Каталожный номер | HT319012 |
|---|---|
| Описание |
Recombinant Human SULT1B1 Protein, N-His (HT319012) expressed in E. coli, spanning Met1-Ile296. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | O43704 |
| Длина белка | Met1-Ile296 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Homo sapiens (Human) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 36.93 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | Sulfotransferase 1B1, ST1B1, 2.8.2.1, Sulfotransferase 1B2, Sulfotransferase family cytosolic 1B member 1, Thyroid hormone sulfotransferase, SULT1B1, ST1B2, SULT1B2 |
| Фон | Sulfotransferase 1B1 (SULT1B1) is a ~34 kDa protein. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of dopamine, small phenols such as 1-naphthol and p-nitrophenol and thyroid hormones, including 3,3'-diiodothyronine, triidothyronine (T3) and reverse triiodothyronine (rT3). May play a role in gut microbiota-host metabolic interaction. O-sulfonates 4-ethylphenol (4-EP), a dietary tyrosine-derived metabolite produced by gut bacteria. The product 4-EPS crosses the blood-brain barrier and may negatively regulate oligodendrocyte maturation and myelination, affecting the functional connectivity of different brain regions associated with the limbic system. 1. Tibbs, ZE. et al. (2018) Xenobiotica; the fate of foreign compounds in biological systems 48, 79-88. PMID: 28084139 2. Fujita, K. et al. (1997) Journal of biochemistry 122, 1052-61. PMID: 9443824 3. Wang, J. et al. (1998) Molecular pharmacology 53, 274-82. PMID: 9463486 4. Needham, BD. et al. (2022) Nature 602, 647-653. PMID: 35165440 |
| Примечание | For research use only. |

SDS-PAGE for Recombinant Human SULT1B1 Protein, N-His
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