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| カタログ番号 | HC862012 |
|---|---|
| 説明 |
Recombinant Human SUPT5H Protein, N-His (HC862012) expressed in E. coli, spanning Lys274-Val644. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O00267 |
| タンパク質長 | Lys274-Val644 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 44.75 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, DSIF large subunit, DSIF p160, SPT5, SPT5H, SUPT5H, Tat-CT1 protein, Tat-cotransactivator 1 protein, Transcription elongation factor SPT5, hSPT5 |
| 背景 | Transcription elongation factor SPT5 is a ~121 kDa protein. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. 1. Yamaguchi, Y. et al. (1999) The Journal of biological chemistry 274, 8085-92. PMID: 10075709 2. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401 3. Wen, Y. et al. (1999) Genes & development 13, 1774-9. PMID: 10421630 4. Ivanov, D. et al. (2000) Molecular and cellular biology 20, 2970-83. PMID: 10757782 5. Wada, T. et al. (2000) Molecular cell 5, 1067-72. PMID: 10912001 6. Ping, YH. et al. (2001) The Journal of biological chemistry 276, 12951-8. PMID: 11112772 7. Renner, DB. et al. (2001) The Journal of biological chemistry 276, 42601-9. PMID: 11553615 8. Kim, DK. et al. (2003) Genes to cells : devoted to molecular & cellular mechanisms 8, 371-8. PMID: 12653964 9. Kwak, YT. et al. (2003) Molecular cell 11, 1055-66. PMID: 12718890 10. Mandal, SS. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 7572-7. PMID: 15136722 |
| 注意事項 | For research use only |
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