Human SYK Recombinant Protein (N-His) (HW747012)

Name: Human SYK Recombinant Protein (N-His)
Brand: abinScience
SKU: HW747012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	HW747012
Descripción	Recombinant Human SYK Protein, N-His (HW747012) expressed in E. coli, spanning Asp374-Asn635. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P43405
Longitud de proteína	Asp374-Asn635
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	32.58 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Spleen tyrosine kinase, SYK, Tyrosine-protein kinase SYK, p72-Syk
Fondo	Tyrosine-protein kinase SYK is a ~72 kDa protein. Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. 1. Urzainqui, A. et al. (2002) Immunity 17, 401-12. PMID: 12387735 2. Wang, L. et al. (2021) Nature genetics 53, 500-510. PMID: 33782605 3. Chiu, CW. et al. (2002) The EMBO journal 21, 6461-72. PMID: 12456653 4. Shim, EK. et al. (2004) FEBS letters 575, 35-40. PMID: 15388330 5. M Gagné, L. et al. (2021) The Journal of biological chemistry 297, 101291. PMID: 34634301 6. Law, CL. et al. (1996) Molecular and cellular biology 16, 1305-15. PMID: 8657103 7. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Human SYK Recombinant Protein (N-His)

Referencias

Datasheet

Human SYK Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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