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Human TAF2 Recombinant Protein (N-His) (HD371012)

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概要
カタログ番号HD371012
説明
Recombinant Human TAF2 Protein, N-His (HD371012) expressed in E. coli, spanning Tyr26-Gly527. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
発現システムE. coli
アクセッション番号Q6P1X5
タンパク質長Tyr26-Gly527
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Homo sapiens (Human)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
予測分子量 59.75 kDa
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名150 kDa cofactor of initiator function, CIF150, RNA polymerase II TBP-associated factor subunit B, TAF(II)150, TAF2, TAF2B, TAFII-150, TAFII150, TBP-associated factor 150 kDa, Transcription initiation factor TFIID 150 kDa subunit, Transcription initiation factor TFIID subunit 2
背景

Transcription initiation factor TFIID subunit 2 is a ~136 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF2 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF1.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Kaufmann, J. et al. (1998) Molecular and cellular biology 18, 233-9. PMID: 9418870
3. Martinez, E. et al. (1998) Molecular and cellular biology 18, 6571-83. PMID: 9774672
注意事項 For research use only
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