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Human TBP Recombinant Protein (N-His) (HB847032)

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Aperçu
Numéro de catalogueHB847032
Description
Recombinant Human TBP Protein, N-His (HB847032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsP20226
Longueur de protéineGly160-Thr339
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsGTF2D1, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, TATA-box-binding protein, TBP, TF2D, TFIID, Transcription initiation factor TFIID TBP subunit
Contexte

TATA-box-binding protein is a ~37 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Kao, CC. et al. (1990) Science (New York, N.Y.) 248, 1646-50. PMID: 2194289
3. Peterson, MG. et al. (1990) Science (New York, N.Y.) 248, 1625-30. PMID: 2363050
4. Hoffman, A. et al. (1990) Nature 346, 387-90. PMID: 2374612
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Louder, RK. et al. (2016) Nature 531, 604-9. PMID: 27007846
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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