Human TET3 Recombinant Protein (N-His) (HC899012)

Name: Human TET3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC899012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HC899012
Description	Recombinant Human TET3 Protein, N-His (HC899012) expressed in E. coli, spanning Val835-Gln1140. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O43151
Longueur de protéine	Val835-Gln1140
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	36.61 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	CXXC10, EC:1.14.11.80, KIAA0401, Methylcytosine dioxygenase TET3, TET3
Contexte	Methylcytosine dioxygenase TET3 is a ~193 kDa protein. Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. 1. Beck, DB. et al. (2020) American journal of human genetics 106, 234-245. PMID: 31928709 2. Xu, Y. et al. (2012) Cell 151, 1200-13. PMID: 23217707 3. Deplus, R. et al. (2013) The EMBO journal 32, 645-55. PMID: 23353889 4. Xu, C. et al. (2018) Structure (London, England : 1993) 26, 85-95.e3. PMID: 29276034
Note	For research use only

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Human TET3 Recombinant Protein (N-His).pdf

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