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| カタログ番号 | HC899012 |
|---|---|
| 説明 |
Recombinant Human TET3 Protein, N-His (HC899012) expressed in E. coli, spanning Val835-Gln1140. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O43151 |
| タンパク質長 | Val835-Gln1140 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 36.61 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CXXC10, EC:1.14.11.80, KIAA0401, Methylcytosine dioxygenase TET3, TET3 |
| 背景 | Methylcytosine dioxygenase TET3 is a ~193 kDa protein. Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. 1. Beck, DB. et al. (2020) American journal of human genetics 106, 234-245. PMID: 31928709 2. Xu, Y. et al. (2012) Cell 151, 1200-13. PMID: 23217707 3. Deplus, R. et al. (2013) The EMBO journal 32, 645-55. PMID: 23353889 4. Xu, C. et al. (2018) Structure (London, England : 1993) 26, 85-95.e3. PMID: 29276034 |
| 注意事項 | For research use only |
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