中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HA072012 |
|---|---|
| 説明 |
Recombinant Human TIGAR Protein, N-His (HA072012) expressed in E. coli, spanning Met1-Arg270. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9NQ88 |
| タンパク質長 | Met1-Arg270 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 32.23 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5, TP53-induced glycolysis regulatory phosphatase |
| 背景 | Fructose-2,6-bisphosphatase TIGAR is a ~30 kDa protein. Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. 1. Li, H. et al. (2009) The Journal of biological chemistry 284, 1748-54. PMID: 19015259 2. Bensaad, K. et al. (2006) Cell 126, 107-20. PMID: 16839880 3. Wanka, C. et al. (2012) The Journal of biological chemistry 287, 33436-46. PMID: 22887998 4. Bensaad, K. et al. (2009) The EMBO journal 28, 3015-26. PMID: 19713938 5. Cheung, EC. et al. (2013) Developmental cell 25, 463-77. PMID: 23726973 6. Ye, L. et al. (2013) Biochemical and biophysical research communications 437, 300-6. PMID: 23817040 7. Cheung, EC. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 20491-6. PMID: 23185017 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TIGAR Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
