中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HD816012 |
|---|---|
| 説明 |
Recombinant Human TMEM175 Protein, N-GST & C-His (HD816012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9BSA9 |
| タンパク質長 | Ser2-Ser33&Ala161-His184&Arg231-Ser257&Ala397-His416&Leu470-Cys504 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Endosomal/lysosomal proton channel TMEM175, Potassium channel TMEM175, TMEM175, Transmembrane protein 175, hTMEM175 |
| 背景 | Endosomal/lysosomal proton channel TMEM175 is a ~55 kDa protein. Proton-activated proton channel that catalyzes proton efflux from endosomes and lysosomes to maintain a steady-state pH. Activated at low pH (under pH 4.6) by luminal side protons: selectively mediates lysosomal proton release from lysosomes, eliciting a proton leak that balances V-ATPase activity to maintain pH homeostasis. Regulation of lumenal pH stability is required for autophagosome-lysosome fusion. Also acts as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes. Constitutes the pore-forming subunit of the lysoK(GF) complex, a complex activated by extracellular growth factors. 1. Zheng, W. et al. (2022) Science advances 8, eabm1568. PMID: 35333573 2. Hu, M. et al. (2022) Cell 185, 2292-2308.e20. PMID: 35750034 3. Zhang, J. et al. (2023) Molecular cell 83, 2524-2539.e7. PMID: 37390818 4. Cang, C. et al. (2015) Cell 162, 1101-12. PMID: 26317472 5. Brunner, JD. et al. (2020) eLife 9. PMID: 32267231 6. Lee, C. et al. (2017) Nature 547, 472-475. PMID: 28723891 7. Oh, S. et al. (2020) eLife 9. PMID: 32228865 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
