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Human TL1A/TNFSF15 Recombinant Protein (C-His) (HF526011)

Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
Human TL1A/TNFSF15 Recombinant Protein (C-His)
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  • 1mg
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Aperçu
Numéro de catalogueHF526011
Description
Recombinant Human TNFSF15/TL1A Protein, C-His (HF526011) expressed in Mammalian Cells, spanning Ala71-Leu251. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionMammalian cells
Numéro d'accèsO95150
Longueur de protéineAla71-Leu251
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Applications testées WB
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 23.37 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsTumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1, Vascular endothelial cell growth inhibitor, Tumor necrosis factor ligand superfamily member 15, membrane form, Tumor necrosis factor ligand superfamily member 15, secreted form, TNFSF15, TL1, VEGI
Contexte

Tumor necrosis factor ligand superfamily member 15 (TNFSF15/TL1A) is a ~28 kDa protein. Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis.

Note For research use only.
Images
  • Human TL1A/TNFSF15 Recombinant Protein (C-His)

    Bioactivity

    Detected by Research Grade Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Antibody (AMG 966) (Cat No.: HF526066) inindirectELISA.

  • Human TL1A/TNFSF15 Recombinant Protein (C-His)

    Bioactivity

    Detected by Anti-Human TNFSF15/TL1A Antibody (3B3) (Cat No.: HF526023) in indirect ELISA.

  • Human TL1A/TNFSF15 Recombinant Protein (C-His)

    Bioactivity

    Detected by Research Grade Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Antibody (AMG 966) (Cat No.: HF526066) in indirect ELISA.

  • Human TL1A/TNFSF15 Recombinant Protein (C-His)

    Western blot

    Detected by Anti-TNFSF15/TL1A monoclonal antibody (Catalog No: HF526066)

  • Human TL1A/TNFSF15 Recombinant Protein (C-His)

    Western blot

    Detected by Anti-TNFSF15/TL1A monoclonal antibody (Catalog No: HF526023)

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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