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| カタログ番号 | HC342012 |
|---|---|
| 説明 |
Recombinant Human TP53BP1 Protein, N-His (HC342012) expressed in E. coli, spanning Ala1614-His1972. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q12888 |
| タンパク質長 | Ala1614-His1972 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 41.86 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | TP53-binding protein 1, TP53BP1, p53-binding protein 1, p53BP1, 53BP1 |
| 背景 | TP53-binding protein 1 (TP53BP1) is a ~213 kDa protein. Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. 1. Wang, B. et al. (2002) Science (New York, N.Y.) 298, 1435-8. PMID: 12364621 2. Botuyan, MV. et al. (2006) Cell 127, 1361-73. PMID: 17190600 3. Kang, Y. et al. (2011) Biochemical and biophysical research communications 404, 476-81. PMID: 21144835 4. Chapman, JR. et al. (2012) Journal of cell science 125, 3529-34. PMID: 22553214 5. Escribano-Díaz, C. et al. (2013) Molecular cell 49, 872-83. PMID: 23333306 6. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538 7. Drané, P. et al. (2017) Nature 543, 211-216. PMID: 28241136 8. Bigot, N. et al. (2019) eLife 8. PMID: 31135337 9. Swift, ML. et al. (2023) Nature structural & molecular biology 30, 1456-1467. PMID: 37696958 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human TP53BP1 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
