Human TRAF4 Recombinant Protein (N-His) (HV470012)

Name: Human TRAF4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV470012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HV470012
Description	Recombinant Human TRAF4 Protein, N-His (HV470012) expressed in E. coli, spanning Gln193-Asp444. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9BUZ4
Longueur de protéine	Gln193-Asp444
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	30.71 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	CART1, MLN62, RING finger protein 83, TRAF4, MLN 62, Cysteine-rich domain associated with RING and Traf domains protein 1, RNF83, Metastatic lymph node gene 62 protein, TNF receptor-associated factor 4
Contexte	TNF receptor-associated factor 4 (TRAF4) is a ~53 kDa protein. Adapter protein with E3 ligase activity that is involved in many diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation or apoptosis. Promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation. Ubiquitinates SMURF2 through 'Lys-48'-linked ubiquitin chain leading to SMURF2 degradation through the proteasome and subsequently osteogenic differentiation. Promotes 'Lys-63'-mediated ubiquitination of CHK1 which in turn activates cell cycle arrest and activation of DNA repair. In addition, promotes an atypical 'Lys-29'-linked ubiquitination at the C-terminal end of IRS1 which is crucial for insulin-like growth factor (IGF) signal transduction. 1. Cai, G. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 11531-11536. PMID: 30352854 2. Li, J. et al. (2019) Cell death and differentiation 26, 2652-2666. PMID: 31076633 3. Cen, S. et al. (2020) EBioMedicine 54, 102722. PMID: 32268273 4. Yu, W. et al. (2021) The Journal of biological chemistry 296, 100739. PMID: 33991522 5. Yu, X. et al. (2020) Journal of hematology & oncology 13, 40. PMID: 32357935
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human TRAF4 Protein, N-His

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Human TRAF4 Recombinant Protein (N-His).pdf

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