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| カタログ番号 | HD347012 |
|---|---|
| 説明 |
Recombinant Human TRIM65 Protein, N-His (HD347012) expressed in E. coli, spanning Val319-Ser517. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q6PJ69 |
| タンパク質長 | Val319-Ser517 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 24.79 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, TRIM65, Tripartite motif-containing protein 65 |
| 背景 | E3 ubiquitin-protein ligase TRIM65 is a ~57 kDa protein. E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation. Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs. This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy. Upon enteroviral infection, promotes 'Lys-63'-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade. Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs. 1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402 2. Tang, T. et al. (2021) Frontiers in immunology 12, 741839. PMID: 34512673 3. Li, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 6970-5. PMID: 24778252 4. Pan, X. et al. (2019) Cell death & disease 10, 429. PMID: 31160576 5. Kato, K. et al. (2021) Molecular cell 81, 599-613.e8. PMID: 33373584 6. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649 |
| 注意事項 | For research use only |
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