Human TRIM65 Recombinant Protein (N-His) (HD347012)

Name: Human TRIM65 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD347012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HD347012
Описание	Recombinant Human TRIM65 Protein, N-His (HD347012) expressed in E. coli, spanning Val319-Ser517. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q6PJ69
Длина белка	Val319-Ser517
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	24.79 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, TRIM65, Tripartite motif-containing protein 65
Фон	E3 ubiquitin-protein ligase TRIM65 is a ~57 kDa protein. E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation. Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs. This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy. Upon enteroviral infection, promotes 'Lys-63'-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade. Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs. 1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402 2. Tang, T. et al. (2021) Frontiers in immunology 12, 741839. PMID: 34512673 3. Li, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 6970-5. PMID: 24778252 4. Pan, X. et al. (2019) Cell death & disease 10, 429. PMID: 31160576 5. Kato, K. et al. (2021) Molecular cell 81, 599-613.e8. PMID: 33373584 6. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649
Примечание	For research use only

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Human TRIM65 Recombinant Protein (N-His).pdf

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