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| カタログ番号 | HF525012 |
|---|---|
| 説明 |
Recombinant Human TRPA1 Protein, N-His (HF525012) expressed in E. coli, spanning Asp63-Ile370. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O75762 |
| タンパク質長 | Asp63-Ile370 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 36.18 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Transformation-sensitive protein p120, TRPA1, Transient receptor potential cation channel subfamily A member 1, Ankyrin-like with transmembrane domains protein 1, ANKTM1, Wasabi receptor |
| 背景 | Transient receptor potential cation channel subfamily A member 1 (TRPA1) is a ~127 kDa protein. Ligand-activated Ca(2+)-permeable, nonselective cation channel involved in pain detection and possibly also in cold perception, oxygen concentration perception, cough, itch, and inner ear function. Has a relatively high Ca(2+) selectivity, with a preference for divalent over monovalent cations (Ca(2+) > Ba(2+) > Mg(2+) > NH4(+) > Li(+) > K(+)), the influx of cation into the cytoplasm leads to membrane depolarization. Has a central role in the pain response to endogenous inflammatory mediators, such as bradykinin and to a diverse array of irritants. Activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds, such as allylthiocyanate (AITC) from mustard oil or wasabi, cinnamaldehyde, diallyl disulfide (DADS) from garlic, and acrolein, an environmental irritant. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner. 1. Zurborg, S. et al. (2007) Nature neuroscience 10, 277-9. PMID: 17259981 2. Bobkov, YV. et al. (2011) Biochimica et biophysica acta 1808, 1120-8. PMID: 21195050 3. Takahashi, N. et al. (2011) Nature chemical biology 7, 701-11. PMID: 21873995 4. Buday, T. et al. (2012) Cough (London, England) 8, 11. PMID: 23199233 5. Moparthi, L. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 16901-6. PMID: 25389312 6. Liu, C. et al. (2021) Neuron 109, 273-284.e4. PMID: 33152265 7. Hu, H. et al. (2009) Nature chemical biology 5, 183-90. PMID: 19202543 8. Kremeyer, B. et al. (2010) Neuron 66, 671-80. PMID: 20547126 |
| 注意事項 | For research use only. |
SDS-PAGE for Human TRPA1 Recombinant Protein (N-His)
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