Human TSC1 Recombinant Protein (N-His) (HV007012)

Name: Human TSC1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV007012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HV007012
Descripción	Recombinant Human TSC1 Protein, N-His (HV007012) expressed in E. coli, spanning Asp998-Ser1164. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q92574
Longitud de proteína	Asp998-Ser1164
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	19.81 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	TSC, Hamartin, Tuberous sclerosis 1 protein, KIAA0243, TSC1
Fondo	Hamartin (TSC1) is a ~129 kDa protein. Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. The TSC-TBC complex acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Within the TSC-TBC complex, TSC1 stabilizes TSC2 and prevents TSC2 self-aggregation. 1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553 2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141 3. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785 4. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059 5. Menon, S. et al. (2014) Cell 156, 771-85. PMID: 24529379 6. Lim, JS. et al. (2017) American journal of human genetics 100, 454-472. PMID: 28215400 10. Woodford, MR. et al. (2017) The EMBO journal 36, 3650-3665. PMID: 29127155
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human TSC1 Protein, N-His

Referencias

Datasheet

Human TSC1 Recombinant Protein (N-His).pdf

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Formula

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