Human TSPAN8 Recombinant Protein (N-GST & C-His) (HB737012)

Name: Human TSPAN8 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HB737012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB737012
Description	Recombinant Human TSPAN8 Protein, N-GST & C-His (HB737012) expressed in E. coli, spanning Lys110-Asn205. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P19075
Longueur de protéine	Lys110-Asn205
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	39.17 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Tetraspanin-8, Tspan-8, Transmembrane 4 superfamily member 3, Tumor-associated antigen CO-029, TSPAN8, TM4SF3
Contexte	Tetraspanin-8 (TSPAN8) is a ~26 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking. Promotes ADAM17-mediated TNF processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration. Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR. 1. Schmidt, F. et al. (2016) Biological chemistry. PMID: 27180357 2. Müller, M. et al. (2022) Cells 11. PMID: 36078095 3. Pan, SJ. et al. (2015) International journal of molecular sciences 16, 5363-74. PMID: 25761241 4. Zhuang, L. et al. (2022) Cell biology international 46, 1693-1703. PMID: 35904232 5. Min, J. et al. (2023) Cellular and molecular life sciences : CMLS 80, 154. PMID: 37204469 6. Zhu, Y. et al. (2023) Cancers 15. PMID: 37835445
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human TSPAN8 Protein, N-GST & C-His

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Human TSPAN8 Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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