Human TUBB3 Recombinant Protein (C-His) (HC498012)

Name: Human TUBB3 Recombinant Protein (C-His)
Brand: abinScience
SKU: HC498012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HC498012
Описание	Recombinant Human TUBB3 Protein, C-His (HC498012) expressed in E. coli, spanning Met1-Lys450. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q13509
Длина белка	Met1-Lys450
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	51.71 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Tubulin beta-III, TUBB4, TUBB3, Tubulin beta-4 chain, Tubulin beta-3 chain
Фон	Tubulin beta-3 chain (TUBB3) is a ~50 kDa protein. Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. TUBB3 plays a critical role in proper axon guidance and maintenance. Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. 1. LaFrance, BJ. et al. (2022) Proceedings of the National Academy of Sciences of the United States of America 119. PMID: 34996871 2. Brito, C. et al. (2024) Science (New York, N.Y.) 383, 870-876. PMID: 38305685 3. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661 4. Tischfield, MA. et al. (2010) Cell 140, 74-87. PMID: 20074521 5. Shao, Q. et al. (2017) The Journal of neuroscience : the official journal of the Society for Neuroscience 37, 5620-5633. PMID: 28483977
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human TUBB3 Protein, C-His

Ссылки

Даташит

Human TUBB3 Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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