Human UBE2A Recombinant Protein (N-His) (HB413012)

Name: Human UBE2A Recombinant Protein (N-His)
Brand: abinScience
SKU: HB413012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB413012
Description	Recombinant Human UBE2A Protein, N-His (HB413012) expressed in E. coli, spanning Met1-Cys152. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P49459
Longueur de protéine	Met1-Cys152
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	19.48 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Ubiquitin-conjugating enzyme E2 A; 2.3.2.23; E2 ubiquitin-conjugating enzyme A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2A; RAD6A
Contexte	Ubiquitin-conjugating enzyme E2 A is a ~17 kDa protein. E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN. 1. Kim, J. et al. (2005) Molecular cell 20, 759-70. PMID: 16337599 2. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386 3. Haddad, DM. et al. (2013) Molecular cell 50, 831-43. PMID: 23685073 4. Hong, JH. et al. (2015) Molecular & cellular proteomics : MCP 14, 674-85. PMID: 25582440 5. Haakonsen, DL. et al. (2024) Nature 626, 874-880. PMID: 38297121 6. Barnsby-Greer, L. et al. (2024) Nature structural & molecular biology 31, 351-363. PMID: 38182926
Note	For research use only

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SDS-PAGE for Recombinant Human UBE2A Protein, N-His

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Human UBE2A Recombinant Protein (N-His).pdf

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