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Human UGT1A1 Recombinant Protein (N-His) (HA604012)

Human UGT1A1 Recombinant Protein (N-His)
Human UGT1A1 Recombinant Protein (N-His)
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  • 100ug
  • 1mg
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Aperçu
Numéro de catalogueHA604012
Description
Recombinant Human UGT1A1 Protein, N-His (HA604012) expressed in E. coli, spanning Gly28-Arg450. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsP22309
Longueur de protéineGly28-Arg450
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 49.69 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsUDP-glucuronosyltransferase 1A1; UGT1A1; 2.4.1.17; Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; UDP-glucuronosyltransferase 1A isoform 1; UGT1A1; GNT1; UGT1
Contexte

UDP-glucuronosyltransferase 1A1 is a ~59 kDa protein. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol. Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates. Involved in the glucuronidation of arachidonic acid (AA) and AA-derived eicosanoids including 15-HETE, 20-HETE, PGB1 and F2-isoprostane (8-iso-PGF2alpha).

1. Gagné, JF. et al. (2002) Molecular pharmacology 62, 608-17. PMID: 12181437
2. Lépine, J. et al. (2004) The Journal of clinical endocrinology and metabolism 89, 5222-32. PMID: 15472229
3. Udomuksorn, W. et al. (2007) Pharmacogenetics and genomics 17, 1017-29. PMID: 18004206
4. Girard, H. et al. (2007) Pharmacogenetics and genomics 17, 1077-89. PMID: 18004212
5. Itäaho, K. et al. (2008) Drug metabolism and disposition: the biological fate of chemicals 36, 2307-15. PMID: 18719240
6. Sneitz, N. et al. (2010) Human mutation 31, 52-9. PMID: 19830808
7. Sneitz, N. et al. (2013) Drug metabolism and disposition: the biological fate of chemicals 41, 582-91. PMID: 23288867
8. Little, JM. et al. (2004) Journal of lipid research 45, 1694-703. PMID: 15231852
9. Li, X. et al. (2011) Drug metabolism and pharmacokinetics 26, 341-50. PMID: 21422672
10. Milne, GL. et al. (2024) Redox biology 70, 103020. PMID: 38211441
Note For research use only
Images
  • Human UGT1A1 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human UGT1A1 Protein, N-His

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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