Human ULK1 Recombinant Protein (N-His) (HF942012)

Name: Human ULK1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF942012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HF942012
Descripción	Recombinant Human ULK1 Protein, N-His (HF942012) expressed in E. coli, spanning Met1-His275. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	O75385
Longitud de proteína	Met1-His275
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	33.40 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, KIAA0722, Serine/threonine-protein kinase ULK1, hATG1, ATG1
Fondo	Serine/threonine-protein kinase ULK1 is a ~112 kDa protein. Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. 1. Chan, EY. et al. (2009) Molecular and cellular biology 29, 157-71. PMID: 18936157 2. Löffler, AS. et al. (2011) Autophagy 7, 696-706. PMID: 21460634 3. Jung, CH. et al. (2011) Autophagy 7, 1212-21. PMID: 21795849 4. Nazio, F. et al. (2013) Nature cell biology 15, 406-16. PMID: 23524951 5. Ro, SH. et al. (2014) The FEBS journal 281, 3816-27. PMID: 25040165 6. Kim, JH. et al. (2018) EMBO reports 19. PMID: 29487085 7. Di Rienzo, M. et al. (2019) Science advances 5, eaau8857. PMID: 31123703
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human ULK1 Protein, N-His

Referencias

Datasheet

Human ULK1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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