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| カタログ番号 | HC297012 |
|---|---|
| 説明 |
Recombinant Human USP10 Protein, N-His (HC297012) expressed in E. coli, spanning Thr600-Leu798. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q14694 |
| タンパク質長 | Thr600-Leu798 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 25.10 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Ubiquitin thioesterase 10, USP10, Ubiquitin carboxyl-terminal hydrolase 10, Ubiquitin-specific-processing protease 10, Deubiquitinating enzyme 10, KIAA0190 |
| 背景 | Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a ~87 kDa protein. Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, RPS2/us5, RPS3/us3, RPS10/eS10, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. 1. Soncini, C. et al. (2001) Oncogene 20, 3869-79. PMID: 11439350 2. Boulkroun, S. et al. (2008) American journal of physiology. Renal physiology 295, F889-900. PMID: 18632802 3. Meyer, C. et al. (2020) Molecular cell 77, 1193-1205.e5. PMID: 31981475 4. Yuan, J. et al. (2010) Cell 140, 384-96. PMID: 20096447 5. Liu, J. et al. (2011) Cell 147, 223-34. PMID: 21962518 6. Kedersha, N. et al. (2016) The Journal of cell biology 212, 845-60. PMID: 27022092 9. Sanders, DW. et al. (2020) Cell 181, 306-324.e28. PMID: 32302570 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human USP10 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
