Human VDR Recombinant Protein (N-His) (HY304012)

Name: Human VDR Recombinant Protein (N-His)
Brand: abinScience
SKU: HY304012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	HY304012
Описание	Recombinant Human VDR Protein, N-His (HY304012) expressed in E. coli, spanning Met272-Ser427. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P11473
Длина белка	Met272-Ser427
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	20.02 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	VDR, Vitamin D3 receptor, 1, 25-dihydroxyvitamin D3 receptor, NR1I1, Nuclear receptor subfamily 1 group I member 1
Фон	Vitamin D3 receptor (VDR) is a ~48 kDa protein. Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis. Also functions as a receptor for the secondary bile acid lithocholic acid (LCA) and its metabolites. 1. Rochel, N. et al. (2000) Molecular cell 5, 173-9. PMID: 10678179 2. Eelen, G. et al. (2005) Molecular pharmacology 67, 1566-73. PMID: 15728261 3. Hourai, S. et al. (2006) Journal of medicinal chemistry 49, 5199-205. PMID: 16913708 4. Tamura, M. et al. (2017) Scientific reports 7, 5102. PMID: 28698609 5. Tsai, JM. et al. (2023) Molecular cell 83, 2753-2767.e10. PMID: 37478846 6. Makishima, M. et al. (2002) Science (New York, N.Y.) 296, 1313-6. PMID: 12016314 7. Hashimoto, N. et al. (2020) Kidney international 97, 1164-1180. PMID: 32354638
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human VDR Protein, N-His

Ссылки

Даташит

Human VDR Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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