中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC127012 |
|---|---|
| 説明 |
Recombinant Human VPS35 Protein, N-His (HC127012) expressed in E. coli, spanning Met1-Asp341. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q96QK1 |
| タンパク質長 | Met1-Asp341 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 41.65 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Vacuolar protein sorting-associated protein 35, hVPS35, Maternal-embryonic 3, Vesicle protein sorting 35, VPS35, MEM3 |
| 背景 | Vacuolar protein sorting-associated protein 35 is a ~91 kDa protein. Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. 1. McGough, IJ. et al. (2018) Nature communications 9, 3737. PMID: 30213940 2. Arighi, CN. et al. (2004) The Journal of cell biology 165, 123-33. PMID: 15078903 3. Vergés, M. et al. (2004) Nature cell biology 6, 763-9. PMID: 15247922 4. Tabuchi, M. et al. (2010) Journal of cell science 123, 756-66. PMID: 20164305 5. Harbour, ME. et al. (2012) The Biochemical journal 442, 209-20. PMID: 22070227 6. McNally, KE. et al. (2017) Nature cell biology 19, 1214-1225. PMID: 28892079 7. Zavodszky, E. et al. (2014) Nature communications 5, 3828. PMID: 24819384 8. McGough, IJ. et al. (2014) Current biology : CB 24, 1670-1676. PMID: 24980502 9. Harbour, ME. et al. (2010) Journal of cell science 123, 3703-17. PMID: 20923837 |
| 注意事項 | For research use only |
SDS-PAGE for Recombinant Human VPS35 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
