Human WAS Recombinant Protein (N-GST & C-His) (HW679022)

Name: Human WAS Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HW679022
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HW679022
Описание	Recombinant Human WAS Protein, N-GST & C-His (HW679022) expressed in E. coli, spanning Gly40-Glu131. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P42768
Длина белка	Gly40-Glu131
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	38.54 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	WASp, WAS, Wiskott-Aldrich syndrome protein, IMD2
Фон	Actin nucleation-promoting factor WAS is a ~52 kDa protein. Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. 1. Cory, GO. et al. (2002) The Journal of biological chemistry 277, 45115-21. PMID: 12235133 2. Cory, GO. et al. (2003) Molecular cell 11, 1229-39. PMID: 12769847 3. Chereau, D. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 16644-9. PMID: 16275905 4. Symons, M. et al. (1996) Cell 84, 723-34. PMID: 8625410 5. Ramesh, N. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 14671-6. PMID: 9405671 6. Cheng, HC. et al. (2008) Nature 454, 1009-13. PMID: 18650809 7. Taylor, MD. et al. (2010) Science translational medicine 2, 37ra44. PMID: 20574068 8. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human WAS Protein, N-GST & C-His

Ссылки

Даташит

Human WAS Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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