Human WAS Recombinant Protein (N-His) (HW679012)

Name: Human WAS Recombinant Protein (N-His)
Brand: abinScience
SKU: HW679012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HW679012
Description	Recombinant Human WAS Protein, N-His (HW679012) expressed in E. coli, spanning Leu39-Gly251. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P42768
Longueur de protéine	Leu39-Gly251
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	25.45 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	WASp, WAS, Wiskott-Aldrich syndrome protein, IMD2
Contexte	Actin nucleation-promoting factor WAS is a ~52 kDa protein. Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. 1. Cory, GO. et al. (2002) The Journal of biological chemistry 277, 45115-21. PMID: 12235133 2. Cory, GO. et al. (2003) Molecular cell 11, 1229-39. PMID: 12769847 3. Chereau, D. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 16644-9. PMID: 16275905 4. Symons, M. et al. (1996) Cell 84, 723-34. PMID: 8625410 5. Ramesh, N. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 14671-6. PMID: 9405671 6. Cheng, HC. et al. (2008) Nature 454, 1009-13. PMID: 18650809 7. Taylor, MD. et al. (2010) Science translational medicine 2, 37ra44. PMID: 20574068 8. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human WAS Protein, N-His

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Human WAS Recombinant Protein (N-His).pdf

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Formula

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