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| カタログ番号 | HV104012 |
|---|---|
| 説明 |
Recombinant Human WNK1 Protein, N-His (HV104012) expressed in E. coli, spanning Lys1954-Ala2231. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9H4A3 |
| タンパク質長 | Lys1954-Ala2231 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 31.88 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | HSN2, Erythrocyte 65 kDa protein, Protein kinase lysine-deficient 1, KDP, p65, hWNK1, KIAA0344, Kinase deficient protein, Protein kinase with no lysine 1, Serine/threonine-protein kinase WNK1, PRKWNK1, WNK1 |
| 背景 | Serine/threonine-protein kinase WNK1 is a ~250 kDa protein. Serine/threonine-protein kinase component of the WNK1-SPAK/OSR1 kinase cascade, which acts as a key regulator of blood pressure and regulatory volume increase by promoting ion influx. WNK1 mediates regulatory volume increase in response to hyperosmotic stress by acting as a molecular crowding sensor, which senses cell shrinkage and mediates formation of a membraneless compartment by undergoing liquid-liquid phase separation. The membraneless compartment concentrates WNK1 with its substrates, OXSR1/OSR1 and STK39/SPAK, promoting WNK1-dependent phosphorylation and activation of downstream kinases OXSR1/OSR1 and STK39/SPAK. Following activation, OXSR1/OSR1 and STK39/SPAK catalyze phosphorylation of ion cotransporters SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A5/KCC2 and SLC12A6/KCC3, regulating their activity. Phosphorylation of Na-K-Cl cotransporters SLC12A2/NKCC1 and SLC12A2/NKCC1 promote their activation and ion influx; simultaneously, phosphorylation of K-Cl cotransporters SLC12A5/KCC2 and SLC12A6/KCC3 inhibit their activity, blocking ion efflux. 1. Lenertz, LY. et al. (2005) The Journal of biological chemistry 280, 26653-8. PMID: 15883153 2. Zagórska, A. et al. (2007) The Journal of cell biology 176, 89-100. PMID: 17190791 3. Jonniya, NA. et al. (2019) ACS omega 4, 17404-17416. PMID: 31656913 4. Schiapparelli, P. et al. (2021) Cell reports 36, 109416. PMID: 34289367 5. Boyd-Shiwarski, CR. et al. (2022) Cell 185, 4488-4506.e20. PMID: 36318922 6. Moriguchi, T. et al. (2005) The Journal of biological chemistry 280, 42685-93. PMID: 16263722 7. Yagi, YI. et al. (2009) Biochemistry 48, 10255-66. PMID: 19739668 8. Richardson, C. et al. (2011) Journal of cell science 124, 789-800. PMID: 21321328 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human WNK1 Protein, N-His
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