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| カタログ番号 | HX078012 |
|---|---|
| 説明 |
Recombinant Human YBX1 Protein, N-GST & C-His (HX078012) expressed in E. coli, spanning Thr57-Thr126. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P67809 |
| タンパク質長 | Thr57-Thr126 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 36.04 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Y-box-binding protein 1, YB-1, CCAAT-binding transcription factor I subunit A, CBF-A, DNA-binding protein B, DBPB, Enhancer factor I subunit A, EFI-A, Nuclease-sensitive element-binding protein 1, Y-box transcription factor, YBX1, NSEP1, YB1 |
| 背景 | Y-box-binding protein 1 (YBX1) is a ~35 kDa protein. DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation. Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C). Promotes mRNA stabilization: acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay. Component of the CRD-mediated complex that promotes MYC mRNA stability. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. 1. Chen, CY. et al. (2000) Genes & development 14, 1236-48. PMID: 10817758 2. Capowski, EE. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 5970-6. PMID: 11698476 3. Gaudreault, I. et al. (2004) Nucleic acids research 32, 316-27. PMID: 14718551 4. Chattopadhyay, R. et al. (2008) Molecular and cellular biology 28, 7066-80. PMID: 18809583 5. Chen, X. et al. (2019) Nature cell biology 21, 978-990. PMID: 31358969 6. Horwitz, EM. et al. (1994) The Journal of biological chemistry 269, 14130-9. PMID: 8188694 7. Ray, D. et al. (2009) Nature biotechnology 27, 667-70. PMID: 19561594 8. Weidensdorfer, D. et al. (2009) RNA (New York, N.Y.) 15, 104-15. PMID: 19029303 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human YBX1 Protein, N-GST & C-His
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