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| カタログ番号 | HD949012 |
|---|---|
| 説明 |
Recombinant Human YTHDC1 Protein, N-His-SUMO & C-Strep (HD949012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q96MU7 |
| タンパク質長 | Ser334-Ser416 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | KIAA1966, Splicing factor YT521, YT521, YT521-B, YTH domain-containing protein 1, YTHDC1 |
| 背景 | YTH domain-containing protein 1 is a ~84 kDa protein. Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. 1. Xu, C. et al. (2014) Nature chemical biology 10, 927-9. PMID: 25242552 2. Xu, C. et al. (2015) The Journal of biological chemistry 290, 24902-13. PMID: 26318451 3. Xiao, W. et al. (2016) Molecular cell 61, 507-519. PMID: 26876937 4. Roundtree, IA. et al. (2017) eLife 6. PMID: 28984244 5. Zhang, Z. et al. (2010) The Journal of biological chemistry 285, 14701-10. PMID: 20167602 6. Patil, DP. et al. (2016) Nature 537, 369-373. PMID: 27602518 |
| 注意事項 | For research use only |
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