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| カタログ番号 | HS805012 |
|---|---|
| 説明 |
Recombinant Human ZC3H12A Protein, N-His (HS805012) expressed in E. coli, spanning Asp426-Glu599. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q5D1E8 |
| タンパク質長 | Asp426-Glu599 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 20.73 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Regnase-1, MCPIP, Endoribonuclease ZC3H12A, Reg1, MCPIP-1, MCP-induced protein 1, ZC3H12A, Zinc finger CCCH domain-containing protein 12A, MCPIP1, Monocyte chemotactic protein-induced protein 1 |
| 背景 | Endoribonuclease ZC3H12A is a ~65 kDa protein. Endoribonuclease involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay. Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation. Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL). Inhibits cooperatively with ZC3H12A the differentiation of helper T cells Th17 in lungs. 1. Mizgalska, D. et al. (2009) The FEBS journal 276, 7386-99. PMID: 19909337 2. Garg, AV. et al. (2015) Immunity 43, 475-87. PMID: 26320658 3. Xu, J. et al. (2012) Nucleic acids research 40, 6957-65. PMID: 22561375 4. Huang, S. et al. (2015) The Journal of biological chemistry 290, 20782-20792. PMID: 26134560 5. Suzuki, HI. et al. (2011) Molecular cell 44, 424-36. PMID: 22055188 6. Roy, A. et al. (2013) American journal of physiology. Cell physiology 305, C1021-32. PMID: 24048733 7. Wang, W. et al. (2015) The Journal of biological chemistry 290, 13372-85. PMID: 25861989 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human ZC3H12A Protein, N-His
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