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Human ZDHHC1 Recombinant Protein (N-His-KSI) (HD005012)

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Vista general
Número de catálogoHD005012
Descripción
Recombinant Human ZDHHC1 Protein, N-His-KSI (HD005012) expressed in E. coli, spanning His263-Ser485. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoQ8WTX9
Longitud de proteínaHis263-Ser485
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 41.60 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosC16orf1, DHHC domain-containing cysteine-rich protein 1, DHHC-1, EC:2.3.1.225, Palmitoyltransferase ZDHHC1, ZDHHC1, ZNF377, Zinc finger DHHC domain-containing protein 1, Zinc finger protein 377
Fondo

Palmitoyltransferase ZDHHC1 is a ~54 kDa protein. Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, such as NCDN and NLRP3. Has a palmitoyltransferase activity toward NCDN and regulates NCDN association with endosome membranes through this palmitoylation. Acts as an activator of the NLRP3 inflammasome by mediating palmitoylation of 'Cys-130' and 'Cys-958' of NLRP3, thereby promoting NLRP3 phosphorylation and activation by NEK7.

1. Nie, L. et al. (2024) Molecular cell 84, 3336-3353.e7. PMID: 39173637
Nota For research use only
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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