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Influenza A virus (H2N2) HA/Hemagglutinin Recombinant Protein (C-His) (VK515021)

Influenza A virus (H2N2) HA/Hemagglutinin Recombinant Protein (C-His)
Influenza A virus (H2N2) HA/Hemagglutinin Recombinant Protein (C-His)
Influenza A virus (H2N2) HA/Hemagglutinin Recombinant Protein (C-His)
Influenza A virus (H2N2) HA/Hemagglutinin Recombinant Protein (C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерVK515021
Описание
Recombinant Influenza A virus (H2N2) HA/Hemagglutinin Protein, C-His (VK515021) expressed in Mammalian Cells, spanning Asp16-Gln525. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииMammalian cells
Номер доступаP03451
Длина белкаAsp16-Gln525
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыInfluenza A virus (H2N2)
Характер Recombinant
Цель HA, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 66.07 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияHA, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Фон

Hemagglutinin (HA) is a ~63 kDa protein. Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

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Примечание For research use only.
Изображения
  • Influenza A virus (H2N2) HA/Hemagglutinin Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Influenza A virus (H2N2) HA/Hemagglutinin Protein, C-His

  • Influenza A virus (H2N2) HA/Hemagglutinin Recombinant Protein (C-His)

    Bioactivity

    Detects VIR-2482 in indirect ELISAs.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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