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MERS-CoV S1-NTD Recombinant Protein (C-His)
MERS-CoV S1-NTD Recombinant Protein (C-His)
MERS-CoV S1-NTD Recombinant Protein (C-His)
MERS-CoV S1-NTD Recombinant Protein (C-His)
Цена(USD):
Спецификация:
  • 50ug
  • 100ug
  • 1mg
Количество:
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  • Обзор

  • Изображения

  • Ссылки

  • Даташит

  • SDS

Обзор
Каталожный номерVK800021
Описание
Recombinant MERS-CoV S/Spike glycoprotein (NTD) Protein, C-His (VK800021) expressed in Mammalian Cells, spanning Met1-Tyr351. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииMammalian cells
Номер доступаK9N5Q8
Длина белкаMet1-Tyr351
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыMiddle East respiratory syndrome-related coronavirus (MERS-CoV)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 40.40 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияSpike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, Spike protein S2, Spike protein S2', S, 3
Фон

Spike glycoprotein (Spike glycoprotein (NTD)) is a ~149 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Interacts with host DPP4 to mediate virla entry.

Примечание For research use only.
Изображения
  • MERS-CoV S1-NTD Recombinant Protein (C-His)

    SDS-PAGE

    SDS-PAGE for Recombinant MERS-CoV S/Spike glycoprotein (NTD) Protein, C-His

  • MERS-CoV S1-NTD Recombinant Protein (C-His)

    Bioactivity

    Detected by Anti-MERS-CoV S1 N-terminal domain/S1-NTD Antibody (7D10) (Cat No.: VK800083) inindirectELISA.

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Рекомендация

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