Recombinant Mouse CD16a/FCGR3A Protein, C-His (MY386011) expressed in Mammalian Cells, spanning Met1-Gln203. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression
Mammalian cells
Numéro d'accès
A0A0B4J1G0
Longueur de protéine
Met1-Gln203
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Mus musculus (Mouse)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
24.05 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs
CD16-II, CD16A, CD16a, CD16a antigen, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, FcgammaRIIIA, IGFR3, IgG Fc receptor III-2, IgG Fc receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-A
Contexte
Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a/FCGR3A) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.
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2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335
3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369
4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922
5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175
6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339
7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325
8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432
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