Recombinant Mouse CD16a/FCGR3A Protein, C-His (MY386011) expressed in Mammalian Cells, spanning Met1-Gln203. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии
Mammalian cells
Номер доступа
A0A0B4J1G0
Длина белка
Met1-Gln203
Применения
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Mus musculus (Mouse)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
24.05 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
CD16-II, CD16A, CD16a, CD16a antigen, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, FcgammaRIIIA, IGFR3, IgG Fc receptor III-2, IgG Fc receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-A
Фон
Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a/FCGR3A) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.
1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607
2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335
3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369
4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922
5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175
6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339
7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325
8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432
中文
English
한국어
日本語
Español
Français
Русский
