Please ensure Javascript is enabled for purposes of website accessibility
Accueil > Products > Protéines Recombinantes > Autres Protéines

Mouse CD289/TLR9 Recombinant Protein (N-His) (MV425012)

Mouse CD289/TLR9 Recombinant Protein (N-His)
Mouse CD289/TLR9 Recombinant Protein (N-His)
Prix(USD) :
Spécification :
  • 50ug
  • 100ug
  • 1mg
Nombre :
Contactez-nous
  • Aperçu

  • Images

  • Reférences

  • Fiche technique

  • SDS

Aperçu
Numéro de catalogueMV425012
Description
Recombinant Mouse CD289/TLR9 Protein, N-His (MV425012) expressed in E. coli, spanning Leu26-Asp421. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionE. coli
Numéro d'accèsQ9EQU3
Longueur de protéineLeu26-Asp421
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesMus musculus (Mouse)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 47.03 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsToll-like receptor 9, CD289, Tlr9
Contexte

Toll-like receptor 9 is a ~116 kDa protein. Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also acts via ADCY7, leading to cyclic di-AMP (c-di-AMP) synthesis and activation of the NLRP3 inflammasome.

1. Khan, NS. et al. (2019) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33, 12500-12514. PMID: 31408613
2. Tabeta, K. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 3516-21. PMID: 14993594
3. Anderson, AE. et al. (2007) European journal of immunology 37, 1548-61. PMID: 17474149
4. Ewald, SE. et al. (2008) Nature 456, 658-62. PMID: 18820679
5. Park, B. et al. (2008) Nature immunology 9, 1407-14. PMID: 18931679
6. Ewald, SE. et al. (2011) The Journal of experimental medicine 208, 643-51. PMID: 21402738
7. Ohto, U. et al. (2015) Nature 520, 702-5. PMID: 25686612
8. Liu, Q. et al. (2025) Nature chemical biology 21, 1283-1291. PMID: 40419769
Note For research use only
Images
  • Mouse CD289/TLR9 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Mouse CD289/TLR9 Protein, N-His

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

Contactez-nous pour les devis personnalisés, les demandes en gros et tout autre problème.

Courrier : [email protected]

Liste des distributeurs