Mouse N-Cadherin Recombinant Protein (N-His) (MB862012)

Name: Mouse N-Cadherin Recombinant Protein (N-His)
Brand: abinScience
SKU: MB862012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	MB862012
Описание	Recombinant Mouse CD325/CDH2 Protein, N-His (MB862012) expressed in E. coli, spanning Asn263-Val491. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P15116
Длина белка	Asn263-Val491
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Mus musculus (Mouse)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	27.04 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Cadherin-2, Neural cadherin, N-cadherin, CD325, Cdh2
Фон	Cadherin-2 is a ~99 kDa protein. Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. Required for proper neurite branching. 1. Cavallaro, U. et al. (2001) Nature cell biology 3, 650-7. PMID: 11433297 2. Yasuda, S. et al. (2007) Neuron 56, 456-71. PMID: 17988630 3. Vendome, J. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, E4175-84. PMID: 25253890 4. Miyatani, S. et al. (1989) Science (New York, N.Y.) 245, 631-5. PMID: 2762814 5. Tamura, K. et al. (1998) Neuron 20, 1153-63. PMID: 9655503 6. Porlan, E. et al. (2014) Nature cell biology 16, 629-38. PMID: 24952463 7. Wang, X. et al. (2016) Scientific reports 6, 19006. PMID: 26750727 8. Gess, B. et al. (2015) Experimental neurology 264, 55-66. PMID: 25496840
Примечание	For research use only

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Mouse CD325/CDH2 Protein, N-His

Ссылки

Даташит

Mouse N-Cadherin Recombinant Protein (N-His).pdf

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