ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Mus musculus (Mouse)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
19.65 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
C-type lectin superfamily member 9, Macrophage-inducible C-type lectin, MINCLE, CLECSF9, CLEC4E, C-type lectin domain family 4 member E
Фон
C-type lectin domain family 4 member E (CLEC4E) is a ~24 kDa protein. Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 (Th1) and T-helper 17 (Th17) cell subtypes. Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation.
1. Wells, CA. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 7404-13. PMID: 18490740
2. Bugarcic, A. et al. (2008) Glycobiology 18, 679-85. PMID: 18509109
3. Yamasaki, S. et al. (2008) Nature immunology 9, 1179-88. PMID: 18776906
4. Yamasaki, S. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 1897-902. PMID: 19171887
5. Ishikawa, E. et al. (2009) The Journal of experimental medicine 206, 2879-88. PMID: 20008526
6. Miyake, Y. et al. (2013) Immunity 38, 1050-62. PMID: 23602766
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Mouse CLEC4E Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
