Mouse Glypican 3 Recombinant Protein (N-His-SUMO) (MW310012)

Name: Mouse Glypican 3 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: MW310012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	MW310012
Descripción	Recombinant Mouse GPC3/Glypican 3 Protein, N-His-SUMO (MW310012) expressed in E. coli, spanning Ile344-Ser553. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q8CFZ4
Longitud de proteína	Ile344-Ser553
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mus musculus (Mouse)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	36.42 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Glypican-3, Glypican-3 alpha subunit, Glypican-3 beta subunit, Gpc3
Fondo	Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. 1. Capurro, MI. et al. (2008) Developmental cell 14, 700-11. PMID: 18477453 2. Bhave, VS. et al. (2013) The American journal of pathology 183, 153-9. PMID: 23665349 3. Song, HH. et al. (2005) The Journal of biological chemistry 280, 2116-25. PMID: 15537637 4. Paine-Saunders, S. et al. (2000) Developmental biology 225, 179-87. PMID: 10964473 5. Grisaru, S. et al. (2001) Developmental biology 231, 31-46. PMID: 11180950 6. Ng, A. et al. (2009) Developmental biology 335, 208-15. PMID: 19733558
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Mouse GPC3/Glypican 3 Protein, N-His-SUMO

Referencias

Datasheet

Mouse Glypican 3 Recombinant Protein (N-His-SUMO).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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