Mouse HTR1A Recombinant Protein (N-His-KSI) (MY581012)

Name: Mouse HTR1A Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: MY581012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	MY581012
Descripción	Recombinant Mouse HTR1A Protein, N-His-KSI (MY581012) expressed in E. coli, spanning Ile218-Lys345. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q64264
Longitud de proteína	Ile218-Lys345
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mus musculus (Mouse)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	29.53 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	5-hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; Serotonin receptor 1A; Htr1a; Gpcr18
Fondo	5-hydroxytryptamine receptor 1A is a ~46 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. HTR1A is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission: signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Beta-arrestin family members regulate signaling by mediating both receptor desensitization and resensitization processes. 1. Ase, AR. et al. (2000) Journal of neurochemistry 75, 2415-26. PMID: 11080193 2. Deng, DR. et al. (2007) Neuroscience 147, 388-402. PMID: 17543467 3. Audero, E. et al. (2008) Science (New York, N.Y.) 321, 130-3. PMID: 18599790 4. Richardson-Jones, JW. et al. (2011) The Journal of neuroscience : the official journal of the Society for Neuroscience 31, 6008-18. PMID: 21508226 5. Charest, A. et al. (1993) The Journal of neuroscience : the official journal of the Society for Neuroscience 13, 5164-71. PMID: 8254366 6. Ramboz, S. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 14476-81. PMID: 9826725
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Mouse HTR1A Protein, N-His-KSI

Referencias

Datasheet

Mouse HTR1A Recombinant Protein (N-His-KSI).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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