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Mouse IGF2 Recombinant Protein (N-His) (MF572012)

Mouse IGF2 Recombinant Protein (N-His)
Mouse IGF2 Recombinant Protein (N-His)
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Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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  • Datasheet

  • SDS

Vista general
Número de catálogoMF572012
Descripción
Recombinant Mouse IGF2 Protein, N-His (MF572012) expressed in E. coli, spanning Ala25-Gln180. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoP09535
Longitud de proteínaAla25-Gln180
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesMus musculus (Mouse)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 20.03 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosInsulin-like growth factor II, IGF-II, Multiplication-stimulating polypeptide, Insulin-like growth factor II, Preptin, Igf2, Igf-2
Fondo

Insulin-like growth factor 2 (IGF2) is a ~20 kDa protein. The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development (Probable). IGF2 is influenced by placental lactogen (Probable). Also involved in tissue differentiation (Probable).

1. Younis, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E2048-E2057. PMID: 29440408
2. Constância, M. et al. (2002) Nature 417, 945-8. PMID: 12087403
3. DeChiara, TM. et al. (1990) Nature 345, 78-80. PMID: 2330056
4. Wilson, EM. et al. (2006) The Journal of biological chemistry 281, 29962-71. PMID: 16901893
5. Younis, S. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 10250-10266. PMID: 32557799
Nota For research use only.
Imágenes
  • Mouse IGF2 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Mouse IGF2 Protein, N-His

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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