Mouse IL12A/IL-12 p35/NKSF1 Recombinant Protein (C-His) (MB936011)

Name: Mouse IL12A/IL-12 p35/NKSF1 Recombinant Protein (C-His)
Brand: abinScience
SKU: MB936011
Price: 298 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	MB936011
Описание	Recombinant Mouse IL12A/IL-12 p35/NKSF1 Protein, C-His (MB936011) expressed in Mammalian Cells, spanning Arg23-Ala215. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	Mammalian cells
Номер доступа	P43431
Длина белка	Arg23-Ala215
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Mus musculus (Mouse)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	25.15 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	IL-12A, NK cell stimulatory factor chain 1, IL12A, CLMF p35, Interleukin-12 subunit alpha, IL-12 subunit p35, NKSF1, Cytotoxic lymphocyte maturation factor 35 kDa subunit
Фон	Interleukin-12 subunit alpha (IL12A/IL-12 p35) is a ~24 kDa protein. Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity. Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes. 1. Mattner, F. et al. (1996) European journal of immunology 26, 1553-9. PMID: 8766560 2. Collison, LW. et al. (2007) Nature 450, 566-9. PMID: 18033300 3. Zheng, XF. et al. (2018) Frontiers in pharmacology 9, 959. PMID: 30197594
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Mouse IL12A/IL-12 p35/NKSF1 Protein, C-His

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Даташит

Mouse IL12A/IL-12 p35/NKSF1 Recombinant Protein (C-His).pdf

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Formula

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Working Solution

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