Mouse IRF1 Recombinant Protein (N-His) (MY164012)

Name: Mouse IRF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: MY164012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	MY164012
Descripción	Recombinant Mouse IRF1 Protein, N-His (MY164012) expressed in E. coli, spanning Trp11-Ser128. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P15314
Longitud de proteína	Trp11-Ser128
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Mus musculus (Mouse)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	16.19 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Interferon regulatory factor 1, IRF-1, Irf1
Fondo	Interferon regulatory factor 1 (IRF1) is a ~37 kDa protein. Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Has an essentail role in IFNG-dependent immunity to mycobacteria. Binds to a consensus sequence in gene promoters. 1. Taniguchi, T. et al. (2001) Annual review of immunology 19, 623-55. PMID: 11244049 2. Kröger, A. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 5-14. PMID: 11846971 3. Romeo, G. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 39-47. PMID: 11846974 4. Honda, K. et al. (2006) Nature reviews. Immunology 6, 644-58. PMID: 16932750 5. Savitsky, D. et al. (2010) Cancer immunology, immunotherapy : CII 59, 489-510. PMID: 20049431 6. Man, SM. et al. (2015) Nature immunology 16, 467-75. PMID: 25774715
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Mouse IRF1 Protein, N-His

Referencias

Datasheet

Mouse IRF1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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