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| カタログ番号 | MY164012 |
|---|---|
| 説明 |
Recombinant Mouse IRF1 Protein, N-His (MY164012) expressed in E. coli, spanning Trp11-Ser128. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P15314 |
| タンパク質長 | Trp11-Ser128 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 16.19 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Interferon regulatory factor 1, IRF-1, Irf1 |
| 背景 | Interferon regulatory factor 1 (IRF1) is a ~37 kDa protein. Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Has an essentail role in IFNG-dependent immunity to mycobacteria. Binds to a consensus sequence in gene promoters. 1. Taniguchi, T. et al. (2001) Annual review of immunology 19, 623-55. PMID: 11244049 2. Kröger, A. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 5-14. PMID: 11846971 3. Romeo, G. et al. (2002) Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 22, 39-47. PMID: 11846974 4. Honda, K. et al. (2006) Nature reviews. Immunology 6, 644-58. PMID: 16932750 5. Savitsky, D. et al. (2010) Cancer immunology, immunotherapy : CII 59, 489-510. PMID: 20049431 6. Man, SM. et al. (2015) Nature immunology 16, 467-75. PMID: 25774715 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Mouse IRF1 Protein, N-His
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