ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces
Mus musculus (Mouse)
Nature
Recombinant
Niveau d'endotoxines
Please contact with the lab for this information.
Pureté
>90% as determined by SDS-PAGE.
Masse moléculaire prédite
20.80 kDa
Forme
Lyophilized
Tampon de stockage
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Peroxidasin homolog is a ~165 kDa protein. Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV/COL4A1 NC1 hexamer. In turns, directly contributes to the collagen IV network-dependent fibronectin/FN and laminin assembly, which is required for full extracellular matrix (ECM)-mediated signaling. Thus, sulfilimine cross-links are essential for growth factor-induced cell proliferation and survival in endothelial cells, an event essential to basement membrane integrity. In addition, through the bromide oxidation, may promote tubulogenesis and induce angiogenesis through ERK1/2, Akt, and FAK pathways. Moreover brominates alpha2 collagen IV chain/COL4A2 at 'Tyr-1480' and leads to bromine enrichment of the basement membranes.
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2. Lázár, E. et al. (2015) Free radical biology & medicine 83, 273-82. PMID: 25708780
3. Bhave, G. et al. (2017) American journal of physiology. Renal physiology 313, F596-F602. PMID: 28424209
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5. Kim, HK. et al. (2019) International journal of molecular sciences 20. PMID: 31817535
6. He, C. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 15827-15836. PMID: 32571911
7. Bathish, B. et al. (2020) The Journal of biological chemistry 295, 12697-12705. PMID: 32675287
8. Yan, X. et al. (2014) Human molecular genetics 23, 5597-614. PMID: 24895407
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