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| カタログ番号 | MV750012 |
|---|---|
| 説明 |
Recombinant Mouse PXDN Protein, N-His (MV750012) expressed in E. coli, spanning Cys37-Asp194. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q3UQ28 |
| タンパク質長 | Cys37-Asp194 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Mus musculus (Mouse) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 20.80 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EC:1.11.2.-, KIAA0230, MG50, Melanoma-associated antigen MG50, PRG2, PXD01, PXDN, PXDN active fragment, Peroxidasin 1, Peroxidasin homolog, VPO, VPO1, Vascular peroxidase 1, hsPxd01, p53-responsive gene 2 protein |
| 背景 | Peroxidasin homolog is a ~165 kDa protein. Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV/COL4A1 NC1 hexamer. In turns, directly contributes to the collagen IV network-dependent fibronectin/FN and laminin assembly, which is required for full extracellular matrix (ECM)-mediated signaling. Thus, sulfilimine cross-links are essential for growth factor-induced cell proliferation and survival in endothelial cells, an event essential to basement membrane integrity. In addition, through the bromide oxidation, may promote tubulogenesis and induce angiogenesis through ERK1/2, Akt, and FAK pathways. Moreover brominates alpha2 collagen IV chain/COL4A2 at 'Tyr-1480' and leads to bromine enrichment of the basement membranes. 1. Bhave, G. et al. (2012) Nature chemical biology 8, 784-90. PMID: 22842973 2. Lázár, E. et al. (2015) Free radical biology & medicine 83, 273-82. PMID: 25708780 3. Bhave, G. et al. (2017) American journal of physiology. Renal physiology 313, F596-F602. PMID: 28424209 4. Bathish, B. et al. (2018) Archives of biochemistry and biophysics 646, 120-127. PMID: 29626421 5. Kim, HK. et al. (2019) International journal of molecular sciences 20. PMID: 31817535 6. He, C. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 15827-15836. PMID: 32571911 7. Bathish, B. et al. (2020) The Journal of biological chemistry 295, 12697-12705. PMID: 32675287 8. Yan, X. et al. (2014) Human molecular genetics 23, 5597-614. PMID: 24895407 |
| 注意事項 | For research use only |
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